Part:BBa_J72037:Design

{Bmll5} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

PCR

Construction of MED7 Basic Part Bmll5
PCR MLL3/MLL78 on pBca9523-Bmll3          (324bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144#5                  (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-Bmll5  
-------------------------------------
MLL3  Forward Biobricking of Bmll5  CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA
MLL78  Reverse Biobricking of Bmll5  CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG